Journal article
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
S Holmes, HJ Kirkwood, R Bean, K Giewekemeyer, AV Martin, M Hadian-Jazi, MO Wiedorn, D Oberthür, H Marman, L Adriano, N Al-Qudami, S Bajt, I Barák, S Bari, J Bielecki, S Brockhauser, MA Coleman, F Cruz-Mazo, C Danilevski, K Dörner Show all
Nature Communications | Published : 2022
Abstract
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiatio..
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Awarded by Australian Research Council Centre of Excellence in Advanced Molecular Imaging
Funding Acknowledgements
We acknowledge European XFEL in Schenefeld, Germany, for provision of X-ray free electron laser beam-time at Scientific Instrument SPB/SFX and use of the XBI biological sample preparation laboratory, enabled by the XBI User Consortium and would like to thank the instrument group and facility staff for their assistance and everyone who attended the first experiment. We would also like to acknowledge the funding support for B.A., C.D, K.A.N, M. H-J and S.H from Australian Research Council Centre of Excellence in Advanced Molecular Imaging (CE140100011), www.imagingcoe.org and the Australian Nuclear Science and Technology Organisation (ANSTO); the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron, part of ANSTO, and funded by the Australian Government. M.L.S., M.A.C. and M.F. performed, in part, under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344. M.L.S., M.A.C. and M.F. were supported by the NIH grant 1R01GM11734201. I.B acknowledges support byMinistry of Education, Science, Research and Sport of the Slovak Republic and by grant from the Slovak Research and Development Agency under contract APVV-14-0181. M.L.S., M.A.C. andM.F. performed, inpart, under theauspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DEAC52-07NA27344. M.L.S., M.A.C. and M.F. were supported by the NIH grant 1R01GM11734201. T.V is a recipient of ARC DECRA (DE170100783). B.K work is funded by National Health and Medical Research Council (NHMRC) grants 1107804 and 1160570 to BK and TV; BK is Australian Research Council (ARC) Laureate Fellow (FL180100109). F.C-M and A.M.G-C acknowledge the Spanish Agencia Estatal de Investigacion (grant no. PID2019-108278RB), and the Junta de Andalucia (grant no. P18FR-3375). S.Bari acknowledged the Helmholtz Initiative and Networking Fund through the Young Investigators Program and the Deutsche Forschungsgemeinschaft SFB755/B03. PLX acknowledges a fellowship from the Joachim Herz Stiftung. Additional support was provided by the Cluster of Excellence 'CUI: Advanced Imaging of Matter' of the Deutsche Forschungsgemeinschaft (DFG) -EXC 2056 -project ID 390715994 and by the Human Frontiers Science Program (RGP0010/2017).